Quantitative validation of immunofluorescence and lectin staining using reduced CLARITY acrylamide formulations
نویسندگان
چکیده
منابع مشابه
Immunofluorescence staining of paraffin sections: creating DAB staining like virtual digital images using CMYK color conversion.
Crystal violet treatment of formalin fixed paraffin embedded tissue slides greatly reduces the endogenous autofluorescence, and allows immunofluorescence (IF) staining with FITC or Alexa488 conjugated antibodies. Using cold CCD camera to capture the fluorescence images makes this staining method very sensitive. Here we show that combination of IF with the simultaneous recording of crystal viole...
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15 صفحه اولAlternate gram staining technique using a fluorescent lectin.
Fluorescence-labeled wheat germ agglutinin binds specifically to N-acetylglucosamine in the outer peptidoglycan layer of gram-positive bacteria. The peptidoglycan layer of gram-negative bacteria is covered by a membrane and is not labeled by the lectin. By exploiting this phenomenon, an alternative Gram staining technique has been developed.
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ژورنال
عنوان ژورنال: Brain Structure and Function
سال: 2017
ISSN: 1863-2653,1863-2661
DOI: 10.1007/s00429-017-1583-z